TABLE 2.
Numbers and lengths of PCR-amplified 16S-23S ISRs obtained with primers 16S/4 and 23S/7 from 175 strains of LAB isolated from food
| Species or subspecies | Reference strain(s) | No. of isolates | No. of ISR PCR products | Length(s) of PCR product(s) containing ISR (bp) | |
|---|---|---|---|---|---|
| Carnobacterium | divergens | NCDO 2763T | 14 | 3 | 400, 500, 600 |
| Carnobacterium | piscicola | NCDO 2762T | 24 | 3 | 600, 700, 800 |
| Carnobacterium | gallinarum | NCDO 2766T | 2 | 3 | 600, 700, 800 |
| Carnobacterium | mobile | DSM 4849T | 2 | 3 | 400, 500, 600 |
| Enterococcus | faecium | ATCC 19434 | 2 | 2 | 550, 650 |
| Enterococcus | faecalis | CIP 103015, JH 22 | 2 | 2 | 450, 600 |
| Lactobacillus | sakei | ATCC 15531, ATCC 15521, ATCC 3453 | 12 | 2 | 450, 650 |
| Lactobacillus | acidophilus | ATCC 4356 | 5 | 2 | 450, 650 |
| Lactobacillus | alimentarius | DSM 20181 | 8 | 2 | 450, 650 |
| Lactobacillus | brevis | CIP 102806T, ATCC 3548 | 17 | 2 | 450, 650 |
| Lactobacillus | buchneri | ATCC 9460 | 2 | 2 | 450, 650 |
| Lactobacillus | curvatus | DSM 20019, CIP 102986T | 7 | 2 | 450, 650 |
| Lactobacillus | delbrueckuii subsp. bulgaricus | CIP 101027 | 2 | 2 | 450, 650 |
| Lactobacillus | delbrueckuii subsp. lactis | CIP 101028 | 2 | 2 | 450, 650 |
| Lactobacillus | farciminis | DSM 20180 | 3 | 2 | 450, 650 |
| Lactobacillus | kefir | ATCC 35411 | 6 | 2 | 450, 650 |
| Lactobacillus | helveticus | CIP 103146T | 4 | 2 | 450, 650 |
| Lactobacillus | kefiranofaciens | ATCC 43761 | 2 | 2 | 450, 650 |
| Lactobacillus | parakefiri | CIP 104242T | 2 | 2 | 450, 650 |
| Lactobacillus | kefirgranum | CIP 104241T | 2 | 2 | 450, 650 |
| Lactobacillus | casei | ATCC 11578T | 4 | 2 | 450, 650 |
| Lactobacillus | pentosus | ATCC 8041 | 2 | 2 | 450, 650 |
| Lactobacillus | plantarum | ATCC 14917, NCDO 343 | 19 | 2 | 450, 650 |
| Lactobacillus | graminis | CIP 105164T | 1 | 2 | 450, 650 |
| Lactococcus | lactis subsp. lactis | ATCC 11454 | 3 | 1 | 500 |
| Lactococcus | lactis subsp. cremoris | CNRZ 117 | 3 | 1 | 500 |
| Leuconostoc | mesenteroides subsp. mesenteroides | DSM 20200, DSM 20240, DSM 20241 | 8 | 1 | 600 |
| Leuconostoc | mesenteroides subsp. dextranicum | DSM 20484 | 4 | 1 | 600 |
| Leuconostoc | pseudomesenteroides | DSM 20193 | 2 | 1 | 600 |
| Leuconostoc | lactis | DSM 20192 | 2 | 1 | 600 |
| Weissella | confusa | DSM 20186 | 2 | 3 | 450, 550, 650 |
| Streptococcus | thermophilus | CNRZ 216 | 5 | 1 | 550 |
a
DSM, Deutsche Sammlung von Microorganismen and Zelkulturen GmbH, Braunschweig, Germany; NCDO, National Collection of Dairy Organisms, Reading, United Kingdom; ATCC, American Type Culture Collection, Rockville, Md.; CNRZ, Centre National de Recherches Zootechniques, Jouy-en-Josas, France; CIP, Collection de l'Institut Pasteur, Paris, France.