FIG. 2.

Growth, lactic acid production, slopes, and fluxes for MBP71. Stationary-phase cultures of MBP71 were washed and inoculated at an OD₆₀₀ of 0.3 AU into four 200-ml LM17 cultures at 30°C. For three of the cultures protein synthesis was blocked at 0, 1.5, and 3 h by addition of 100 mg of chloramphenicol (Cam) per liter to the medium. In a fifth culture MBP71 was inoculated at an OD₆₀₀ of 0.03 AU into LM17 containing thymidine (dT). OD₆₀₀ and the pH were monitored over time. (A) OD₆₀₀. When thymidine was added, the specific growth rate was 1.03 h⁻¹. (B) Lactic acid produced. (C) Rate of lactic acid production. (D) Lactic acid flux. Only data points at which the OD₆₀₀ was less than 1.0 and the pH was more than 5.8 are shown. dw, dry weight.