TABLE 1.
Correlation between OD600 and cell dry weighta
| Inoculum (%) | Harvest time (h)
|
OD600 (AU)
|
Cell dry wt (g/liter)
|
Correlation factor (g[dry wt]/liter/AU)
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample 1 | Sample 2 | Sample 3 | Sample 1 | Sample 2 | Sample 3 | Sample 1 | Sample 2 | Sample 3 | Sample 1 | Sample 2 | Sample 3 | |
| 5 | 0.00 | 6.00 | 23.75 | 0.131 | 0.446 | 0.132 | 0.034 | 0.150 | 0.090 | 0.26 | 0.34 | (0.68) |
| 15 | 0.00 | 4.00 | 24.00 | 0.372 | 1.028 | 0.472 | 0.095 | 0.302 | 0.353 | 0.26 | 0.29 | (0.75) |
| 25 | 0.00 | 3.50 | 24.75 | 0.630 | 1.420 | 1.143 | 0.460 | 0.620 | 0.32 | (0.54) | ||
| 5(+dT)b | 0.00 | 5.83 | 25.00 | 0.125 | 3.375 | 3.087 | 0.038 | 0.960 | 1.033 | 0.30 | 0.28 | 0.33 |
a
The OD600 and cell dry weight were determined at three time points (samples 1, 2, and 3) for both exponentially growing cultures (with thymidine) and nonreplicating cultures of MBP71 in LM17. The correlation factor was determined by dividing the cell dry weight by the OD600. The values in parentheses indicate that cultures had lysed.
b
dT, thymidine.