TABLE 2.
Sugar | Relative lactic acid flux for nonreplicating growth (%)
|
Specific growth rate (h−1) for exponential growth MBP71 | ||
---|---|---|---|---|
MBP71 | MBP89 (low activity) | MBP98 (high activity) | ||
Lactose | 100 | (146) | 169 | 1.01 |
Fructose | 152 | 0.97 | ||
Glucose | 141 | 1.09 | ||
Mannose | 131 | 1.03 | ||
Galactose | 105 | 0.85 | ||
Maltose | 85 | 0.86 | ||
Trehalose | 78 | 0.81 | ||
Cellobiose | 66 | 0.84 |
Stationary-phase cultures of MBP98 in M17 containing thymidine and erythromycin supplemented with 1% sugar were washed and inoculated into M17 supplemented with 1% sugar. The OD600 and pH were determined every hour. From 2.5 to 5.5 h the rates of acid production were practically constant, and they were divided by the cell density at 3 h to obtain the lactic acid flux. The fluxes were expressed relative to the value for an MBP71 culture grown in LM17. The value for MBP89 grown in LM17 was obtained in another experiment. The specific growth rates for MBP71 on the various sugars are indicated. MBP71 was grown in M17 containing thymidine supplemented with 1% sugar. The rates were determined from exponential fits of four or five values (R2 > 0.998). The value in parentheses is from another experiment.