TABLE 1.
Organism and reference(s) | Level in cells
|
Level in supernatant
|
||||||
---|---|---|---|---|---|---|---|---|
F420
|
FO
|
F420
|
FO
|
|||||
μmol/g | μmol/liter | μmol/g | μmol/liter | μmol/g | μmol/liter | μmol/g | μmol/liter | |
S. flocculus | ||||||||
This work | 0.62 | 4.43 | 0.056 | 0.515 | 0.37 | 2.95 | 0.28 | 2.58 |
Ref. 9 | 0.023 | 0.069 | NRb | NR | NR | NR | NR | NR |
M. smegmatis | 0.30 | 1.43 | 0.020 | 0.103 | 0.04 | 0.20 | 0.25 | 1.24 |
M. phlei | 0.17 | 0.95 | 0.003 | 0.017 | 0.04 | 0.24 | 0.15 | 0.83 |
A. kijaniata | 0.17 | 0.78 | 0.006 | 0.025 | 0.20 | 0.95 | 0.05 | 0.23 |
S. avermitilis | 0.16 | 0.59 | 0.010 | 0.034 | 0.10 | 0.28 | 0.15 | 0.45 |
R. opacus Rb1 | 0.19 | 0.46 | 0.020 | 0.045 | 0.09 | 0.19 | 0.81 | 1.75 |
A. missouriensis | 0.19 | 0.25 | 0.021 | 0.027 | 0 | 0 | 0.02 | 0.01 |
R. rhodochrous | 0.11 | 0.20 | 0.008 | 0.150 | 0 | 0 | 0.55 | 1.00 |
N. simplex | 0.08 | 0.15 | 0.006 | 0.012 | 0 | 0 | 0.47 | 0.90 |
S. coelicolor | ||||||||
This work | 0.04 | 0.12 | 0.003 | 0.095 | 0.38 | 1.15 | 0.10 | 0.33 |
Ref. 13 | 0.06 | 0.19 | NR | NR | NR | NR | NR | NR |
S. griseus | ||||||||
Ref. 9 | 0.021 | 0.063 | NR | NR | NR | NR | NR | NR |
Ref. 16 | 0.058 | 0.17 | NR | NR | NR | NR | NR | NR |
M. thermoautotrophicum | ||||||||
This workc | 1.7 | 0.85 | 0.03 | 0.02 | NDd | ND | ND | 7.2e |
Refs. 10, 11f | 2.0 | 1.0 | NR | NR | NR | NR | NR | 8.3g |
Methanosarcina barkeri | ||||||||
Refs. 8, 17h | 0.76 | 2.6 | NR | NR | NR | NR | NR | NR |
M. formicicum | ||||||||
Refs. 17, 36i | 1.2 | 0.60 | NR | NR | NR | NR | NR | NR |
Methanococcus, voltae | ||||||||
Refs. 17, 36i | 0.034 | 0.017 | NR | NR | NR | NR | NR | NR |
Refs. 8, 17j | 0.15 | 0.073 | NR | NR | NR | NR | NR | NR |
For growth in flasks, unless noted otherwise. Deazaflavin levels are expressed as micromoles per gram (dry weight) of cells or micromoles per liter of culture. Ref., reference.
NR, not reported.
Cells were fermentor grown in this laboratory, and 5-deazaflavin levels were measured by HPLC.
ND, not determined.
From reference 22.
Values are averages of two determinations by Eirich et al. (10, 11) with fermentor-grown cells, assuming that the dry weight of cells equals 1/7 the wet weight of cells and assuming a culture density of 0.5 g (dry weight) of cells per liter. One determination was made by absorbance of purified material, and the other was made by enzyme assay.
From references 8, 17, and 34.
Grown on methanol; volumetric production was estimated by assuming a yield of 1.7 g (dry weight) of cells per liter in a fed bottle.
Grown on formate in bottles; volumetric production was estimated for a pH-controlled fermentor by assuming a yield of 0.5 g (dry weight) of cells per liter.
Grown on H2 plus CO2 in bottles; volumetric production was estimated by assuming a fermentor yield of 0.5 g (dry weight) of cells per liter.