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. 2002 Nov;68(11):5750–5755. doi: 10.1128/AEM.68.11.5750-5755.2002

TABLE 1.

F420 and FO levels in cells and culture supernatantsa

Organism and reference(s) Level in cells
Level in supernatant
F420
FO
F420
FO
μmol/g μmol/liter μmol/g μmol/liter μmol/g μmol/liter μmol/g μmol/liter
S. flocculus
    This work 0.62 4.43 0.056 0.515 0.37 2.95 0.28 2.58
    Ref. 9 0.023 0.069 NRb NR NR NR NR NR
M. smegmatis 0.30 1.43 0.020 0.103 0.04 0.20 0.25 1.24
M. phlei 0.17 0.95 0.003 0.017 0.04 0.24 0.15 0.83
A. kijaniata 0.17 0.78 0.006 0.025 0.20 0.95 0.05 0.23
S. avermitilis 0.16 0.59 0.010 0.034 0.10 0.28 0.15 0.45
R. opacus Rb1 0.19 0.46 0.020 0.045 0.09 0.19 0.81 1.75
A. missouriensis 0.19 0.25 0.021 0.027 0 0 0.02 0.01
R. rhodochrous 0.11 0.20 0.008 0.150 0 0 0.55 1.00
N. simplex 0.08 0.15 0.006 0.012 0 0 0.47 0.90
S. coelicolor
    This work 0.04 0.12 0.003 0.095 0.38 1.15 0.10 0.33
    Ref. 13 0.06 0.19 NR NR NR NR NR NR
S. griseus
    Ref. 9 0.021 0.063 NR NR NR NR NR NR
    Ref. 16 0.058 0.17 NR NR NR NR NR NR
M. thermoautotrophicum
    This workc 1.7 0.85 0.03 0.02 NDd ND ND 7.2e
    Refs. 10, 11f 2.0 1.0 NR NR NR NR NR 8.3g
Methanosarcina barkeri
    Refs. 8, 17h 0.76 2.6 NR NR NR NR NR NR
M. formicicum
    Refs. 17, 36i 1.2 0.60 NR NR NR NR NR NR
Methanococcus, voltae
    Refs. 17, 36i 0.034 0.017 NR NR NR NR NR NR
    Refs. 8, 17j 0.15 0.073 NR NR NR NR NR NR
a

For growth in flasks, unless noted otherwise. Deazaflavin levels are expressed as micromoles per gram (dry weight) of cells or micromoles per liter of culture. Ref., reference.

b

NR, not reported.

c

Cells were fermentor grown in this laboratory, and 5-deazaflavin levels were measured by HPLC.

d

ND, not determined.

e

From reference 22.

f

Values are averages of two determinations by Eirich et al. (10, 11) with fermentor-grown cells, assuming that the dry weight of cells equals 1/7 the wet weight of cells and assuming a culture density of 0.5 g (dry weight) of cells per liter. One determination was made by absorbance of purified material, and the other was made by enzyme assay.

g

From references 8, 17, and 34.

h

Grown on methanol; volumetric production was estimated by assuming a yield of 1.7 g (dry weight) of cells per liter in a fed bottle.

i

Grown on formate in bottles; volumetric production was estimated for a pH-controlled fermentor by assuming a yield of 0.5 g (dry weight) of cells per liter.

j

Grown on H2 plus CO2 in bottles; volumetric production was estimated by assuming a fermentor yield of 0.5 g (dry weight) of cells per liter.