TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant properties | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| TG1 | supE Δhsd thi Δ(lac-proAB) F′[traD36 proAB+lacIqlacZΔM15] | 26 |
| JM109 | recA endA gyrA thi hsdR relA supE Δ(lac-proAB) | 29 |
| L. lactis | ||
| MG1363 | Derivative of NCDO712, plasmid free, prophage cured | 12 |
| CHCC377 | Industrial strain | Chr Hansen A/S |
| NZ9800 | Tn5276 ΔnisA, Str | 21 |
| UKLc10 | pepN::nisRK | 27 |
| UKLc11 | MG1363 leuB::nisRK, pLP712 | This work |
| UKLc11-I | UKLc11, sex factor telB::pepI | This work |
| UKLc11-Q | UKLc11, sex factor telB::pepQ | This work |
| UKLc11-L | UKLc11, sex factor telB::pepL | This work |
| UKLc11-G | UKLc11, sex factor telB::pepG | This work |
| UKLc11-W | UKLc11, sex factor telB::pepW | This work |
| UKLc11-C | UKLc11, sex factor telB::pepC | This work |
| UKLc13(pLP712) | MG1363(pLP712), sex factor telB::nisRK | This work |
| UKLc13-I(pLP712) | UKLc13(pLP712), sex factor telB::nisRK-pepI | This work |
| Plasmids | ||
| pGEM-T | Apr, Plac-lacZα, linearized, with 3′T overhangs, ColE1 replicon | Promega |
| pBluescriptIISK | Apr, Plac-lacZα, ColE1 replicon | Stratagene |
| pUC18E | pUC18 with 1.2-kb ermG (Err)-containing fragment from L. reuteri in SmaI site | U. Wegmann, personal communication |
| pNZ9573 | Cmr Err, pepN::nisRK, p15a replicon | 5 |
| pG+host9 | Err, pWV01 replicon (temperature sensitive) | 23 |
| pLP712 | lac+prt+, 33 MDa | 12 |
| pJKC4 | Kmr, pepC, pSC105 replicon | 18 |
| pUK200 | Cmr, PnisA, terminator of brnQ, pSH71 replicon | 27 |
| pUK200I | pUK200, PnisA::pepI | 27 |
| pUK200Q | pUK200, PnisA::pepQ | 27 |
| pUK200L | pUK200, PnisA::pepL | 27 |
| pUK200G | pUK200, PnisA::pepG | 27 |
| pUK200W | pUK200, PnisA::pepW | 27 |
| pUK200C | pUK200, PnisA::pepC | This work |
| pUK300 | pG+host9 with ′telA-telB-telC′ from lactococcal sex factor interrupted by multiple-cloning site in telB | This work |
| pUK300I | pUK300 with PnisA::pepI-TbrnQ in EcoRV site | This work |
| pUK300Q | pUK300 with PnisA::pepQ-TbrnQ in EcoRV site | This work |
| pUK300L | pUK300 with PnisA::pepL-TbrnQ in EcoRV site | This work |
| pUK300G | pUK300 with PnisA::pepG-TbrnQ in EcoRV site | This work |
| pUK300W | pUK300 with PnisA::pepW-TbrnQ in EcoRV site | This work |
| pUK300C | pUK300 with PnisA::pepC-TbrnQ in EcoRV site | This work |
| pUK500 | pUK300 with nisRK in ClaI site | This work |
| pUK500I | pUK500 with PnisA::pepI-TbrnQ in SmaI site downstream of nisRK | This work |