Figure 7.

hBex2 regulates NSCL2 activity through formation of transcriptional complex. (A) Endogenous expression of NSCL2 in human fetal brain to confirm the specificity of its polyclonal antibody. (B and C) Interaction of hBex2 and LMO2 with NSCL2, respectively, in human fetal brain tissue. The fetal brain tissue was used to immunoprecipitate the hBex2 (B) or LMO2 (C) with specific polyclonal Abs. Precipitate was blotted with anti-NSCL2 Ab. (D) hBex2 regulates E-box-dependent gene expression through LMO2 and NSCL2. The reporter system used here contains three tandem repeat sequences of wild-type E-box motif adjacent to the firefly luciferase. The mutant reporter gene contains an inactive form of E-box motif as a control. Cos-7 cells were transiently transfected with reporter gene plasmids along with expression plasmids as indicated. A renilla luciferase plasmid was cotransfected in each transfection to evaluate transfection efficiency. All experiments were repeated more than three times with triplication for each experiment. Error bars indicate standard deviations.