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Cells from the experiment described in Figure 4 were collected, fixed in ethanol and stained with propidium iodide. DNA content was determined using flow cytometry. While wild-type cells were able to resume DNA synthesis by 12 h after drug removal, apn1 mutants remained firmly fixed in S-phase. The vertical line serves as a reference for the cell cycle position seen in the culture following 12 h of aminopterin treatment.
