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. 2005 Dec 2;1(6):e61. doi: 10.1371/journal.pgen.0010061

Figure 4. Synthetic Lethality Between dna2 and pol3–01 .

Figure 4

Strain W303 dna2–1 carrying a TRP1 CEN3 DNA2 plasmid was transformed with an integrating URA3 pol3–01 plasmid [120] cut with BamH1. The transformants were streaked on 5-FOA medium to excise the WT POL3 gene and identify clones with the pol3–01 mutation. Three transformants carrying the pol3–01 mutation (isolates 2, 8, and 11) were restreaked on YPD medium containing 2-amino-5-fluorobenzoic acid (FAA), which selects for strains that have lost the DNA2 TRP1–containing plasmid [121]. Three dna2–1 pol3–01 colonies showing some residual growth on the FAA plates were restreaked on YPD-containing medium. The same three isolates of dna2–1 pol3–01 but containing the DNA2 TRP plasmid, i.e., that had not been grown in the presence of FAA, are shown as controls, as indicated.