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. 2005 Dec 6;3(1):e2. doi: 10.1371/journal.pmed.0030002

Figure 3. Validation of Gene Array Results by Q-PCR.

Figure 3

Genes selected for validation by Q-PCR in endothelial cells (A) and fibroblasts (B). MCP-1, ICAM-1, E-selectin, and VCAM-1 transcripts were increased by 12.89-, 25.36-, 17.59-, and 5.86-fold, respectively, in endothelial cells incubated with the antibodies for 4 h compared with control endothelial cells (A). MCP-1, ICAM-1, IL-6, and IL-8 transcripts were increased 5.7-, 3.44-, 9.54-, and 8.22-fold in fibroblasts incubated with the antibodies compared to control fibroblasts (B). The level of transcript expression is reported on the vertical axis. GAPDH was selected as endogenous gene.