TABLE 3.
Group-specific primers based on 16S rDNA sequences
| Target bacteria | Primera | Sequence (5′ to 3′) | Product size (bp) | Annealing temp (°C) |
|---|---|---|---|---|
| Bacteroides fragilis group | g-Bfra-F | ATAGCCTTTCGAAAGRAAGAT | 501 | 50 |
| g-Bfra-R | CCAGTATCAACTGCAATTTTA | |||
| Prevotella | g-Prevo-F | CACRGTAAACGATGGATGCC | 527-529 | 55 |
| g-Prevo-R | GGTCGGGTTGCAGACC | |||
| Bifidobacterium | g-Bifid-F | CTCCTGGAAACGGGTGG | 549-563 | 55 |
| g-Bifid-R | GGTGTTCTTCCCGATATCTACA | |||
| Clostridium coccoides group | g-Ccoc-F | AAATGACGGTACCTGACTAA | 438-441 | 50 |
| g-Ccoc-R | CTTTGAGTTTCATTCTTGCGAA |
a
The standardized primer names are as follows: g-Bfra-F, S-G-Bfra-0148-a-S-21; g-Bfra-R, S-G-Bfra-0626-a-A-21; g-Prevo-F, S-G-Prevo-0803-a-S-20; g-Prevo-R, S-G-Prevo-1311-a-A-16; g-Bifid-F, S-G-Bifid-0153-a-S-17; g-Bifid-R, S-G-Bifid-0699-a-A-22; g-Ccoc-F, S-G-Ccoc-0477-a-S-20; and g-Ccoc-R, S-G-Ccoc-0895-a-A-22 (1).