Figure 3.

Staining of ectopic postsynaptic structures for utrophin and dystrophin. (A) No difference in the staining intensity for utrophin was seen when dsRNA was omitted. (B) Although AChR clusters were formed on the muscle that contained dsRNA_utrophin (GFP-positive), these clusters were often devoid of any utrophin. (C) Dystrophin was more uniformly distributed along the entire plasmalemmal membrane, with only some enrichment at AChR clusters. (D) In dsRNA_utrophin-expressing muscle fibres, dystrophin was found at sites of AChR accumulation. (E) Quantification of the amount of utrophin (green bars) and dystrophin (blue bars) in neighbouring (light colours) and injected muscle fibres (dark colours). Bars in (A–D)=5 μm.