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. 2004 Jan 23;5(2):213–218. doi: 10.1038/sj.embor.7400074

Figure 2.

Figure 2

Elimination of hydrogen peroxide by DJ-1. (A) In all, 3 nM of hydrogen peroxide was reacted with 0.5 nM of DJ-1 for 1 h at 30°C, and the concentration of hydrogen peroxide was measured as described in Methods. (B) In all, 3 nM of hydrogen peroxide was reacted with various amounts of wild-type, V51A and C53A DJ-1 for 1 h at 30°C, and the concentrations of hydrogen peroxide were measured as described in Methods. (C) SHSY-5Y cells in 10-cm dishes were transfected with 5 μg each of plasmids used for the establishment of cell lines by the lipofectamine method. At 36 h after transfection, cells were treated with 10 μM hydrogen peroxide for 60 min and then with 5 μM DCFH-DA for 30 min, and analysed by flow cytometry. BSA, bovine serum albumin.