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. 2004 Jan 23;5(2):172–177. doi: 10.1038/sj.embor.7400078

Figure 2.

Figure 2

IL-12p40 promoter activation by TLR agonists. (A) RAW264.7 cells were transiently transfected with an IL-12p40 promoter reporter plasmid. After 24 h, cells were stimulated with 100 nM CpG-DNA, 100 ng/ml LPS or 30 μg/ml LTA for 8 h. Subsequently, reporter gene activation was measured and is expressed relative to nonstimulated cells. Three independent transfections were performed. (B) RAW264.7 cells stably expressing the IL-12p40 promoter reporter plasmid were stimulated as above for the indicated time periods. (C) The same cells as in (B) were assayed for endogenous IL-12p40 protein after 24 h of stimulation.