FIG. 2.
Integration of pGIP014 into the chromosome of MD007 at the tRNASer locus by site-specific integration. pGIP014 harbors the attP site, which is identical to the chromosomally localized attB site. The int gene product catalyzes the recombination event between the attB and attP sites. The indicated primers tRNA and 40930L1 were used to identify the recombination event. The final strain was designated MD007::pGIP014. In this strain, the alr gene is orientated opposite to all neighboring genes, preventing readthrough expression from surrounding genes.