. 2002 Nov;68(11):5663–5670. doi: 10.1128/AEM.68.11.5663-5670.2002

TABLE 1.

Strains, plasmids and primers used in this study^a

Strain, plasmid, or primer	Relevant features^b	Source of reference
Strains
L. plantarum^a
MD007	L. plantarum NCIMB8826 Δalr,d-Ala auxotroph	M. Deghorain, unpublished data
MD007Int6	Em^r, nisRK, pMEC10 chromosomal integration in MD007 at tRNA^Ser	This work
MD007::pGIP014	Em^r, nisRK, pGIP014 chromosomal integration in MD007 at tRNA^Ser	This work
L. lactis
NZ3900	MG1363 with a chromosomal insertion of nisRK	12
PH3960	NZ3900 Δalr, nisRK	29
Plasmids
pJDC9	Em^r	10
pGIP009	Em^r, pJDC9 derivative containing L. lactis alr gene	This work
pGIT032	Em^r Cm^r, P_ldhL	18
pNZ2650	Cm^r, P_nisA	29
pGIP011	Em^r Cm^r, pGIT032 derivative containing L. lactis alr downstream of P_ldhL	This work
pGIP012	Cm^r, pNZ2650 derivative containing L. lactis alr downstream of P_nisA	This work
pNG8048-Cre	Cm^r	7
pUC-NcoI	Ap^r, pUC18 derivative containing NcoI and ribosome-binding site of prtP	20
pNZ7110	Ap^r, pUC derivative containing cre and T_pepN	This work
pNZ7115	Ap^r, pNZ7110 with cre replaced by L. plantarum alr PCR product	This work
PNZ8020	Cm^r, P_nisA	11
pGIP013	pNZ8020 derivative containing L. plantarum alr from pNZ7115	This work
pGEMT-easy	Ap^r	Promega
pGIP010	Ap^r, pGEMT-easy derivative containing L. lactis alr PCR product	This work
pMEC10	Em^r, nisRK	38
pGIP014	Em^r, nisRK, pMEC10 derivative containing alr fragment of pGIP010	This work
Primers
LLALR-5	5′-CGAGGATCCGCATAGTAATTTAGAAGCTGTTGC-3′	This work
LLALR-11	5′-ACGCGTCGACATTTGTAAAGGCTTTTATGAGAT-3′	This work
LLALR-12	5′-GGGGTACCATTTGTAAAGGCTTTTATGAGAT-3′	This work
LLALR-13	5′-CGGAATTCATTACAGCTCCAAGACTAGTC-3′	This work
TRNA	5′-GCGAACCGGCTAATACCGGC-3′	This work
409301L1	5′-AACAGAAGGTGGGACAGTAG-3′	This work
LplAlr_F	5′-AGGCAATTTGCCATGGTTGTAATTGG-3′	This work
LplAlr_R	5′-ACGTTCTAGATTAATCTATATAAACTCTCGG-3′	This work

Strain NCIMB8826 was obtained from the National Collections of Industrial, Food and Marine Bacteria, Aberdeen, Scotland.

Ap^r, ampicillin resistant; Cm^r, chloramphenicol resistant; Em^r, erythromycin resistant. Underscored base pairs in primers indicate introduced restriction sites.