Figure 4.

The PYM–Mago–Y14 complex is conserved across species. (A) The interaction surfaces of D. melanogaster PYM and the Mago–Y14 heterodimer have been opened up relative to the view in Fig 2A. The two surfaces are coloured according to sequence conservation, ranging from orange for conserved residues to white for variable residues. On the right, the atomic model of PYM is shown bound to the surface of Mago–Y14. (B) Lysates prepared from E. coli expressing untagged H. sapiens (Hs) PYM were incubated with glutathione agarose beads coated with GST, GST–Hs Y14, GST–Hs Mago or GST–Hs Y14–Mago dimers.