FIG. 2.
S(0)T (a) and cell density and (H2S)T (b) over time in triplicate growing cultures of D. desulfuricans ND132, in pH 7.3 medium with 28 mM sulfate (ionic strength = 0.2 M), in the absence (•) and presence (▴) of elemental sulfur. Open circles and triangles indicate killed controls for each group. Concentration of S(0)T was measured by the triphenylphosphine derivatization with high-performance liquid chromatography separation and UV absorbance detection. Error bars represent the standard deviations of measurements.