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FtsK-dependent Xer recombination requires DNA adjacent to the XerD-binding site only. The schematic illustrates reactions between short, labelled dif-containing DNAs and unlabelled DNAs with either (1) two arms flanking dif or one arm adjacent to dif on either the XerD side (2) or the XerC side (3). Recombination reactions were incubated at 37°C for 60 min and analysed by 7% TBE–PAGE.
