Figure 3.

Asymmetric DNA requirement of FtsK_50C is independent of DNA sequence. (A) Orientation of dif sites in pFX315 and pFX316, and the recombination products expected from reactions between dif-containing restriction fragments and a short, labelled dif-containing DNA (3CD3). Lowercase letters indicate DNA orientation. The single RAG sequence element is annotated. (B) Electrophoretic analysis of 60 min reactions detailed in (A). (C) Recombination over time for reactions between short, dif-containing DNAs where either one or both short fragments have a Dside extension. The reactions with just one D-side arm are between 3CD3 and 315 M/N.