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. 2004 Mar 19;5(4):378–384. doi: 10.1038/sj.embor.7400121

Figure 3.

Figure 3

RAD21/SCC1 associates with chromosomal arms and centromeres during meiotic prophase I. Schematic on the far left depicts the major elements of chromosome arm and centromere behaviour in temporal order in meiotic prophase I. Chromosome spreads of mouse spermatocytes were immunostained using antibodies against SCP3 (purple), CREST (staining for centromeric proteins, red) and RAD21/SCC1 (green). The merged images have the following colours for colocalization: RAD21/SCC1 and CREST (yellow); RAD21/SCC1 and SCP3 (vivid blue); and all three proteins (light blue). Representative cells in the five major stages of meiotic prophase I are shown in temporal order: (A) leptonema, with arrows indicating a typical cluster of centromeres; (B) late zygonema; (C) pachynema, with arrows indicating the XY chromosomes (note the absence of RAD21/SCC1 staining on the XY chromosomes); (D) diplonema, with an arrow indicating the site of a crossover; and (E) diakinesis (note that more SCP3 and RAD21/SCC1 are retained on the X chromosomes) (arrows). Detailed views of chromosomes (far right; not to scale) were obtained from the white boxes on the main panels, and are shown from left to right in the order of: SCP3; RAD21/SCC1 alone; RAD21/SCC1 and CREST merged; RAD21/SCC1, SCP3 and CREST merged. Scale bar, 10 μm.