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. 2004 Apr 8;5(5):497–502. doi: 10.1038/sj.embor.7400128

Figure 4.

Figure 4

Accumulation of Rad52–YFP foci in asf1Δ cells. Rad52–YFP foci were visualized by fluorescence microscopy in asynchronous cultures of BY4741 (wt) and Y11310 (asf1Δ) cells transformed with plasmid pWJ1344. (A) Fluorescence and brightfield microscopy of asf1Δ cells expressing the Rad52–YFP fusion protein. (B) Percentage of unbudded (G1) and budded (S and G2/M) wild-type and asf1Δ cells containing Rad52–YFP foci. The total numbers of analysed cells were 55 and 199, and 62 and 396 for unbudded and budded wild-type and asf1Δ cells, respectively.