Skip to main content
. 2004 Apr 23;5(5):490–496. doi: 10.1038/sj.embor.7400139

Figure 4.

Figure 4

HP1α is displaced from its chromatin targets by metK9, metK9–pS10 and metK9–acK14, but not by metK9–pS10–acK14 peptide. (A–N) Fixed NIH3T3 cells were incubated with recombinant GST–HP1α in the presence of the indicated peptides. Cells were then labelled with anti-GST (A–G) and DAPI (H–N). (O) 1 μg of the indicated peptides was spotted on a nitrocellulose membrane and stained with Ponceau (bottom panel). The membrane was then blocked with bovine serum albumin and incubated first with GST–HP1α and then with anti-GST antibodies (top panel). (a–l) Indicated peptides were microinjected into the nuclei of an NIH3T3-derived cell line expressing GFP–HP1β. Injected cells were then photographed every 15 s. Scale bars, 10 μm.