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Caspase-2 stimulates cytochrome c release from Bax−/−,Bak−/− MEFs. Wild-type (WT) or Bax,Bak-deficient cells were harvested, digitonin-permeabilized and incubated with 75 or 100 ng of wild-type caspase-2 for 10 min at 22°C. Supernatant and pellet fractions were separated by SDS–PAGE and western blotted.
