Skip to main content
. 2004 May 28;5(6):620–625. doi: 10.1038/sj.embor.7400166

Figure 2.

Figure 2

The 5′UTR of BACE1 is responsible for reduced BACE1 expression. (A) HEK293 cells were transiently transfected with BACE1, or BACE1 constructs containing the 5′UTR or 3′UTR of BACE1 as shown. Protein levels were corrected for secretory alkaline phosphatase activity. (B) Reduction of BACE1 expression is not cell-type specific. H4 and COS7 cells were transfected with BACE1 or 5′UTR BACE1 and analysed by western blotting (upper panels). GFP was used as a loading control (lower panels). (C) Influence of the BACE1 5′UTR on the expression of a chimeric luciferase (Luc) construct. HEK293 cells were transfected with luciferase containing the 5′UTR of BACE1 or with luciferase as control. The signal for luciferase without 5′UTR was set to 100%. Luciferase activity was corrected for secretory alkaline phosphatase activity. Results were expressed as the mean and standard deviation of three independent experiments made in duplicate.