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. 2004 May 28;5(6):620–625. doi: 10.1038/sj.embor.7400166

Figure 4.

Figure 4

Effect of uORF mutations and deletion constructs on the expression levels of BACE1. (A) Schematic representation of the analysed constructs. Open boxes represent the three uORFs in the 5′UTR, which are indicated by numbers. Mutation of the potential start codons from ATG to ATA are indicated by #, whereas deletion constructs are indicated by Δ. (B) HEK293-APP cells were transiently transfected with BACE1 plasmids bearing the indicated uORF mutations, or (C) deletion mutants. Western blots were probed for BACE1 and GFP (upper panels). Representative northern blots (lower panels) showed similar mRNA levels for all analysed constructs compared with actin levels. (D) Quantification of the results in (C) from two independent experiments. BACE1 protein levels were corrected for BACE1 and actin mRNA levels. The columns show the changes in BACE1 translation for the indicated constructs relative to the BACE1 construct containing the full-length, wild-type 5′UTR (set to one). The inset shows an enlargement of the effect of the Δ224–446 mutant compared with the 5′UTR BACE1 construct.