FIG. 2.

Binding of ¹²⁵I-Cry1Ac (A), biotinylated Cry1Ac (B), and biotinylated Cry1Fa (C) toxins to BBMV from YDK (lanes 1) or YHD2 (lanes 2) insects. Toxins were incubated with BBMV proteins (20 μg) for 1 h. Binding reactions were stopped by centrifugation, and washed pellets were separated by SDS-PAGE. Gels were dried and autoradiographed (A) or transferred to PVDF filters. Biotinylated Cry1Ac (B) and Cry1Fa (C) were detected by using streptavidin-peroxidase conjugate and enhanced chemiluminescence.