Skip to main content
. 2004 May 28;5(7):692–697. doi: 10.1038/sj.embor.7400164

Figure 2.

Figure 2

Mutants deleted in DSK2 and RAD23 are defective in the degradation of the ERAD substrates CPY*HA and CTG*. (A,B) Yeast strains expressing CPY*HA or CTG* were metabolically labelled, chased for the indicated times and immunoprecipitated using anti-HA or anti-CPY antibodies, respectively. Δdsk2 and Δrad23 single- and double-deletion mutants show synergistic effects in ERAD. Degradation of CPY*HA (A) and CTG* (B) is significantly delayed in a Δdsk2Δrad23 strain. Data represent the mean values of three (CPY*HA) and two (CTG*) independent experiments. WT, wild type.