TABLE 3.
Properties of recombinant sMMO-expressing strains
| sMMO-expressing strain (in TJSHM1 expression system) | Activity with naphthalene | Inhibition by acetylene | Added Cu requirement (mg of CuSO4 · 5H2O liter−1) | Culture OD600 during continuous growtha |
|---|---|---|---|---|
| Wild type | Yes | Yes | 0 | 6 |
| C151E | Yes | Yes | 0.1 | 9 |
| C151Y | No | NAb | 0.1 | 9 |
| T213A | Diminished | Yes | 0.1 | 8 |
| T213S | Yes | Yes | 0 | 4 |
a
Strains that could not be maintained without copper during continuous culturing were grown in the presence of 0.1 mg of CuSO4·5H2O liter−1.
b
NA, not applicable.