TABLE 4.
Properties of T213S mutant of sMMO
| Property | Value for:
|
|
|---|---|---|
| Wild type | T213S | |
| Iron content (mol of Fe per mol of 250-kDa hydroxylase)a | 2.74 ± 0.05 | 2.63 ± 0.04 |
| Activity (nmol min−1 mg of hydroxylase−1) with: | ||
| Propeneb | 244 ± 7 | 43 ± 3 |
| Tolueneb | 7.80 ± 0.83 | 5.6 ± 0.49 |
| Methanea,c | 235 ± 22 | 169 ± 27 |
| Product distribution with toluene substrate (mol of benzyl alcohol/mol of p-cresol)b | 2.6 ± 0.8 | 2.8 ± 0.6 |
a
Measurements were conducted in duplicate; values are shown as the mean and standard deviation.
b
Derived from GC quantitation of the product(s). Measurements were performed at least three times each; values are shown as the mean and standard deviation.
c
Derived from substrate-stimulated increase in oxygen uptake as described in Materials and Methods.