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Transcription increases as plastids mature. Fragments of maize plastid genes were either PCR-amplified or isolated from plasmids, separated in agarose gels and blotted onto nylon membranes. Blots were incubated with radiolabelled plastid run-on transcripts from the indicated tissues. The column labelled ‘gel' shows the stained gene fragments before blotting. Tissues tested include leaf sections (1, 5 and 9), and leaf (L) or etiolated coleoptile (Cd) material.
