TABLE 1.
Cytotoxin-hemolysin mRNA detection by RT-PCR in VBNC populations of three strains of V. vulnificus maintained in ASW at 4°C over a 4.5-month period
| Strain |
vvhA mRNA detection in VBNC population of the following age (Tvbnc [days]):a
|
|||||||
|---|---|---|---|---|---|---|---|---|
| 3 | 11 | 18 | 25 | 38 | 67 | 91 | 133b | |
| C7184 | + | + | + | + | + | − | ND | − |
| IF Vv10 | + | + | + | + | W | − | − | − |
| IF Vv18 | + | + | + | + | + | W | + | − |
a
Shown are results of seminested RT-PCR amplifications from a defined quantity of sample. A 25-ml volume of ASW was filtered, and the RNA pellet was dissolved in 25 μl of diethylpyrocarbonate-treated water and diluted twofold for DNase treatment. A 4-μl volume of DNase-treated RNA extract was used for RT. +, bright band; W, faint band; −, no band detected on the gel; ND, not determined.
b
At this stage of the experiment, a weak or a positive result was obtained by concentrating the sample 6- to 12-fold.