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Western blot of protein extracts from wild-type E. coli strain ET8000 grown under nitrogen limitation to induce expression of AmtB from its native promoter. Lane 1 is from a His-tagged construct, while all other lanes are wild type. Lane 1: purified AmtB; lane 2: whole-cell extract; lane 3: cytoplasmic fraction; lane 4: membrane fraction. Samples were separated on a 10% SDS–polyacrylamide gel and visualized with a rabbit polyclonal anti-AmtB antibody.
