Figure 3.

Overexpression of PCS1 prevents normal cell death associated with anther dehiscence. (A,B) An open wt flower (A) showed normal anther dehiscence, whereas the one from a 35S:PCS1–FAST plant (B) failed to dehisce. (C) Anther section from a wt flower before the anther dehiscence process. Septum (SP) and stomium (ST) were not degenerated. (D–F) Anther sections of a wt flower (D) and a 35S:PCS1–HIS flower (E,F) at the flower-opening stage. (F) Close-up view. (G–J) Exogenous ethephon treatments accelerated anther dehiscence of wt flowers (H) but not that of the 35S:PCS1 flowers (I,J). (G) Water-treated wt flower used as a negative control. The flowers in (G–I) were at the stage of 2–3 days before flower opening. The flower shown in (J) was at the stage 2–3 days after flower opening. Magnifications: A,B, × 30; C, × 120; D, × 40; E, × 50; F, × 200; G–I, × 40; J, × 30.