Figure 1.

Amino-acid sequence (single-letter code) alignment of DsRed and Kaede with KikG and a derived mutant, KikGR. The numbering is on the basis of KikG. Regions forming βsheets are indicated by black bars. The residues forming the interior of the β-can fold are shaded. Of these, the residues that were subjected to site-directed random mutagenesis are indicated in dark grey shading. Residues responsible for chromophore synthesis are indicated by asterisks.