TABLE 2.
Synthetic oligonucleotides used in this study
| Oligo- nucleotide | Sequencea |
|---|---|
| RB1 | 5′-TGCTCGAGCAGCAACGCCTACTTAG-3′ |
| RB2 | 5′-TGCTCGAGATATTTGTGCGCTATTGAGACTC-3′ |
| RB3 | 5′-TGCTCGAGTATGAGGCAAATGGATTAGAG-3′ |
| RB4 | 5′-TGCTCGAGGCGGTCGCTTAGATGT-3′ |
| RB5 | 5′-GCTGATGGTTATGGTCTAGCGAATG-3′ |
| RB6 | 5′-TGCGGTGTAGTCATGGCTGTTACT-3′ |
| F1 | 5′-TGCTCGAGTACGGTTCGATTGGCTATTTGAG-3′ |
| F2 | 5′-TGCTCGAGCTTTAAGCGGGTAATGTGATTGA-3′ |
| F3 | 5′-TTGGCGCGCCTGAGCCTAACCAACCCAACCACA-3′ |
| F4 | 5′-TTGGCGCGCCTGAGGACTGCCAATCATAATGTT-3′ |
| F5 | 5′-GGCACTGGCGTCGTCTATC-3′ |
| F6 | 5′-TTTGTTGCGGTATTGAAATTGAAC-3′ |
| F7 | 5′-TTGGCGCGCCAAGCGTTGGGTGGCATCACTTAG-3′ |
| A2 | 5′-TGCTCGAGAAGATACCGCCGTTAGTTTC-3′ |
| A4 | 5′-TTGGCGCGCCAGTGTCGCATTTATTGGCAGA-3′ |
| A7 | 5′-TGGGCGCGCCATCCAGAATCAGGCAATAGCATT-3′ |
| K1 | 5′-ATTGCGATCGATTTATGCTTGTAAACCGTT-3′ |
| AK1 | 5′-TTGGCGCGCCTTATGCTTGTAAACCGTT-3′ |
a
The underlined regions indicate the following restriction endonuclease sites engineered into the oligonucleotides: XhoI sites in RB1, RB2, RB3, RB4, F1, F2, and A2; AscI sites in F3, F4, F7, A7, and AK1; and a ClaI site in K1.