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. 2005 Mar 18;6(4):334–340. doi: 10.1038/sj.embor.7400373

Figure 3.

Figure 3

AVP-induced conformational change of β-arr monitored by intramolecular BRET. HEK293 cells were transfected with the indicated plasmids and BRET was measured at 25°C in the presence of coelenterazine h. (A) Specificity of agonist-induced β-arr intramolecular BRET. (B) Real-time BRET measurements of the agonist-induced β-arr conformational change. t1/2=half-time of maximal conformational change of β-arr. (C) Dose-dependent agonist-promoted increase of β-arr intramolecular BRET. Cells were stimulated with increasing concentrations of AVP for 4 min. EC50=concentration of AVP producing half-maximal conformational change of β-arr. Data are the mean±s.e.m. of at least three independent experiments. *P<0.01 between treated and control condition.