Figure 5.

Double-brilliance β-arr monitors GPCR activation. HEK293 cells were transfected with Luc–β-arr–YFP and either pcDNA3.1 or plasmids encoding the indicated receptors. (A) Agonist-induced translocation of Luc–β-arr–YFP measured following treatment with 1 μM of the specific agonists (β₂-AR, ISO; V1aR, AVP; δ-OR, SNC80; PAFR, PAF; CCR5, hRANTES; AT1aR, angiotensin II). (B) Agonist-induced conformational change of Luc–β-arr–YFP measured following 10 min stimulation with the specific agonists mentioned in (A). Data are the mean±s.e.m. of three independent experiments. ^*P<0.05 between treatment and each individual control condition.