Figure 2.
Nucleosome binding by PRC2 requires Su(z)12 and Nurf55 or Esc. Binding reactions containing 32P-labelled recombinant mononucleosomes (250 fmol) and the indicated recombinant PRC2 subunits/(sub)complexes (200–2,000 fmol; Flag-tagged subunit indicated by ‘F') were resolved on native polyacrylamide gels; position of free mononucleosome is indicated by an arrow. Note that tetrameric PRC2 purified through Flag–Su(z)12 or Flag–E(z) binds with indistinguishable affinity; for further details, see text. A binding reaction containing Flag–E(z)–Su(z)12–Nurf55 was loaded as a reference on some of the gels.