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Sequence analysis of upstream sup-tRNATyr expressed in Escherichia coli. All molecules carry the diagnostic G residue at position 73 (blue, 5′ of the CCA terminus, red) that represents the first position of the downstream RNA. This indicates that in both constructs (sup-tRNATyr-1/T5 tRNACys, sup-tRNATyr-1/HDV), tRNATyr is not released as a 3′-truncated tRNA, but as a complete molecule.
