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. 2005 Apr 15;6(5):458–463. doi: 10.1038/sj.embor.7400390

Figure 3.

Figure 3

Quantification of WPRE-mediated release of DMPK transcripts to the cytoplasm by RT–PCR. (A) Detection of nuclear (N) and cytoplasmic (C) mutant DMPK 3′ UTR transcripts (UTR(200)), in the presence of WPRE or its antisense version (UTR(200W) and UTR(200W-AS), respectively). RNA analysis was carried out from myoblasts or 2- and 6-day differentiated cells (2d Diffn and 6d Diffn, respectively) by amplifying the EGFP gene, which was connected to the mutant DMPK 3′ UTR. As a control, mouse GAPDH cDNA was also amplified. (B) Summary of mutant DMPK 3′ UTR RNA subcellular localization from myoblasts or differentiated cells. In all cases, WPRE (UTR(200W)) caused a significant increase of mutant DMPK 3′ UTR transcripts to the cytoplasm.