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. 2005 Apr 22;6(5):438–444. doi: 10.1038/sj.embor.7400392

Figure 1.

Figure 1

Generation of stable hMRE11-knockdown HeLa cells. Gene silencing of hMRE11 in HeLa cells was achieved by the use of small interfering RNAs. To generate HeLa cells stably displaying hMRE11-knockdown, two pmH1P-neo-based RNAi constructs encoding short hairpin RNAs were used to transfect HeLa cells. Neomycin-resistant clones were selected with 400 μg/ml G418, and 150 μg of cell extracts was analysed by immunoblotting. HeLa, parental control; 12 and 14, two representative stable cell lines.