FIG. 2.

Species specificity of PCR-based assay for P. piscicida. DNAs of dinoflagellate isolates were amplified with specific primers for P. piscicida and actin genes. (Top) NTS1 PCR for P. piscicida with NTS2F and NTS3R primers (256 bp). (Middle) NTS2 PCR for P. piscicida with NTS2F and NTS4R primers (523 bp). (Bottom) Actin-PCR using G-480 and G-482 universal primers (≅730 bp). (−), negative control; (+), positive control with P. piscicida DNA (100 pg) for NTS1 and NTS2 PCR and P. piscicida actin clone (1 ng) for actin-PCR.