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. 2005 Apr 22;6(5):464–469. doi: 10.1038/sj.embor.7400404

Figure 1.

Figure 1

Expression profile of pbmap-2: RT–PCR was performed with RNA from mixed asexual parasites and gametocytes to amplify pbmap-2, lactate dehydrogenase (LDH) and pbs48. pbmap-2 can be amplified robustly from gametocyte complementary DNA but not from asexual-stage cDNA. LDH is expressed in the asexual and sexual stages of the parasite and amplifies readily from both cDNA pools. The gametocyte-specific gene pbs48 gives a strong signal from gametocyte cDNA and a considerably weaker signal from the asexual parasite cDNA sample (possibly because of contamination of asexual parasite preparation by small numbers of gametocytes).