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. 2005 Apr 29;6(5):452–457. doi: 10.1038/sj.embor.7400406

Figure 1.

Figure 1

Interaction of JCV Agno with HP1 in vivo. (A) Schematic representation of human HP1α and HP1γ showing the regions (bars) encoded by cDNA fragments isolated by a yeast two-hybrid assay with the N-terminal region of Agno as a bait. CD and CSD, chromo and chromo-shadow domains, respectively. (B) 293AG cells (HEK293 cells in which the expression of JCV Agno is inducible by Dox) were transfected with a vector for Myc-tagged human HP1α and were incubated in the absence or presence of Dox for 24 h. Cell lysates were subjected to immunoprecipitation with antibodies to Agno (anti-Agno), and the resulting precipitates were subjected to immunoblotting (IB) with anti-Myc. (C) Lysates prepared from 293AG cells after treatment with Dox for 48 h were subjected to immunoprecipitation with anti-HP1α or anti-Agno, and the resulting precipitates and cell lysates (Input) were subjected to immunoblotting with the same antibodies, as indicated. NMS, normal mouse serum; NRS, normal rabbit serum. (D) 293T cells were transfected with a vector for Agno and Myc-tagged human HP1α, β or γ, and were subjected to immunoprecipitation with anti-Agno. The resulting precipitates were subjected to immunoblotting with anti-Myc and anti-Agno.