FIG. 4.

Coomassie blue-stained protein profiles of recombinant E. coli crude extracts separated on sodium dodecyl sulfate-10% polyacrylamide electrophoresis gel. Lane 1 in panels A and B shows molecular size markers as indicated alongside in kilodaltons; the open circles in lanes 2, 3, and 4 in panel A and lanes 3, 4, and 5 in panel B indicate the positions of the overexpressed CpnA, CpnB, CpnBA, CpnC, CpnD, and CpnE proteins in the respective clones pCMP202, pCMP201, pCMP203, pCMP105, pCMP107, and pCMP408 (see Table 1). Lane 5 in panel A and lane 2 in panel B are controls with E. coli cells containing the pSD80 vector only.