TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| Comamonas sp. strain NCIMB 9872 | Grows on cyclopentanol | 27 |
| Comamonas sp. strain 9872MB | cpnB::lacZ, Kmr | This study |
| C. testosteroni ATCC 11996 | Grows on testosterone, type strain | 46 |
| Plasmids | ||
| pKOK6.1 | lacZ-Kmr cassette | 41 |
| pSD80 | Expression vector Apr | 60 |
| pUC18 | Cloning vector, Apr | 77 |
| pUC19 | Cloning vector, Apr | 77 |
| pXcmkn12 | Direct cloning vector of PCR products, Apr Kmr | 14 |
| pMM4 | chnB from Acinetobacter sp strain NCIMB 9871 in pET22b(+) | 17 |
| pCMP10 | 116-bp PCR product in pXcmkn12 | This study |
| pCMP100 | 7.2-kb EcoRI fragment in pUC18 | This study |
| pCMP107 | 1.2-kb SmaI* fragment containing cpnD in pSD80a | This study |
| pCMP105 | 0.9-kb EcoRI*-PstI* fragment containing cpnC in pSD80 | This study |
| pCMP200 | 4.3-kb SphI fragment in pUC18 | This study |
| pCMP201 | 1.7-kb PstI-StuI fragment containing cpnB in pSD80 | This study |
| pCMP202 | 0.8-kb EcoRI*-PstI* fragment containing cpnA in pSD80 | This study |
| pCMP203 | 2.5-kb PstI* fragment containing cpnBA in pSD80 | This study |
| pCMP210 | cpnB::lacZ, Kmr from pCMP200 | This study |
| pCMP300 | 4.8-kb HindIII fragment in pUC18 | This study |
| pCMP400 | 5.7-kb Xhol fragment in pUC19 | This study |
| pCMP408 | 1.5-kb EcoRI* fragment containing cpnE in pSD80 | This study |
a
* indicates restriction sites introduced by PCR design.