FIG. 1.

Galactoside metabolism gene cluster of L. plantarum ATCC 8014. The gene order and nucleotide sequence of intercistronic regions are shown. White arrows correspond to incomplete ORFs. The region cloned in p1α is represented as a bold segment. Relevant restriction enzymes sites are indicated: B, BamHI; E, EcoRI. Promoters (P) and terminators (t) are indicated. Secondary structures are given as corresponding DNA sequences. Stop (∗) and start codons (M) are in bold letters. Promoter sequences (−35, −10) and Shine Dalgarno sequence (SD) are underlined. The CRE site is boxed.