FIG. 2.
Expression of freA, mirA, cycA, acoA, and lysF under standard (+Fe), iron depletion (−Fe), and copper depletion (−Cu) conditions in A. nidulans wild-type (wt) and SREA-deficient (ΔsreA) strains. Fungal strains were grown for 24 h in minimal medium containing 10 μm FeSO4 and 10 μm CuSO4 as described previously (20); for iron- and copper-depleted growth the addition of the respective metal was omitted. As a control for loading and RNA quality, blots were hybridized with the γ-actin-encoding acnA gene (6). (A and B) Northern blot analysis was performed with 1 μg of mRNA (A) or 10 μg of total RNA (B), respectively. (C) Quantification of mRNA levels normalized to acnA levels with a PhosphorImager. Bars represent mean values of two independent experiments; standard deviations did not exceed 20%.