TABLE 1.
Induction of toluene-degradative activity in R. pickettii PKO1 under different culture conditions
| Inducing agent (initial concn) | Growth substrate | Length of incubation period (h)a | Specific toluene degradation rateb (mg of toluene/day/1010 CFU) |
|---|---|---|---|
| None | Lactated | 9 | 0.01 ± 0.01 |
| Spent mediumc | Lactated | 9 | 0.14 ± 0.02 |
| Phenylalanine (>20 mg/liter) | Lactated | 9 | 0.17 ± 0.03 |
| TCE (110 mg/liter) | None | 9 | 0.17 ± 0.03 |
| TCE (110 mg/liter) | Lactated | 9 | 1.31 ± 0.34 |
| Toluene (saturating vapor) | Toluene (saturating vapor) | 72 | 13.05 ± 4.11 |
Steady-state toluene-degradative activity levels were attained within the reported incubation periods.
The specific degradation rate represents the steady-state degradative-activity level. The reported error is the standard deviation of at least three measurements for each culture condition.
The spent medium was collected from 160-ml serum bottles that were incubated for 9 h with lactate (initial concentration, 1,000 mg of sodium dl-lactate per liter) and 220 mg of TCE per liter). The collected spent medium was aseptically purged with air for 30 min and filtered through a 0.2-μm-pore-size membrane to remove remaining TCE and cells from the spent medium.
Initial concentration (1,000 mg of sodium dl-lactate/liter).